The Port Canaveral Lock allows access from the Banana river and Indian river (via the Barge Canal) to the Atlantic Ocean. Built in 1965 it is the largest navigation lock in Florida. The lock was installed to reduce the tidal flow coming through the Port. Hours of operation are 6:30am-9:00pm. Traveling through the locks can take up to 30 minutes, or longer depending on traffic.
There is NO FISHING in or near the Port Canaveral lock. You will not be permitted to use the lock if you are caught fishing.
To contact the Port Canaveral lock, use channel 13 (bridges monitor channel 9) or call 321-783-5421 if you do not have a VHF radio on your vessel. They can also see you on cameras but I wouldn’t depend on that to get passage. The pull cords on either end to signal you want passage have not worked for several years. Let the lock operator know which way you are going (east or west bound passage) and wait for them to cycle the lock. Allow all boats to exit before trying to enter the lock. The lock operator will signal you when its safe to enter/exit the lock. Once you enter the lock, it is best to not tie up completely, leave a little slack and be able to feed line out, the water level can change several feet and you don’t want your vessel hanging from a cleat. Once the lock begins opening, DO NOT try leaving before the lock operator signals you are clear to do so.
Be nice to the lock operators. They are working while you are out enjoying yourself. And they can refuse passage.

There is an area at the lock where you can watch what is happening. The pictures below were taken from there.

West gate open (one side) for passage. All vessels exiting the lock must leave before any may enter for passage the other way.
Port Canaveral Lock
For more information visit the Army Corps of Engineers site.